Journal Covers


Published Book Chapters

Dorrington, R.A., Jiwaji, M., Awando, J.A., and de Bruyn, M.M. (2019). Advances in Tetravirus Research: New Insight into the Infectious Virus Lifecycle and an Expanding Host Range.  In Insect Molecular Virology: Advances and Emerging Trends, B.C. Bonning, ed.  (Norfolk, UK: Caister Academic Press), ISBN: 978-1-912530-09-0. Pp. DOI:

Jiwaji, M, Matcher, GF and RA Dorrington*. (2014) Understanding the unseen majority around us: an overview of microbiological technologies.   In “Bioinformatics and Data Analysis in Microbiology” Tastan-Bishop, O (Ed).  Horizon press. pp 1-23

Matcher, GF,Dorrington, RA and Burton SG* (2012) Enzymatic production of enantiospecific amino acids from mono-substituted hydantoin substrates In “Unnatural Amino Acids”, Vol794, of Methods in Molecular Biology. Pollegioni, L. and Servi, S. (Eds): pp 37-54

Dorrington, RA*, Gorbalenya, AE, Gordon, KHJ, Lauber, C and VK Ward.  (2011) Tetraviridae.  In: “Virus Taxonomy. AMQ King, MJ Adams, EB. Carstens, and Elliot J. Lefkowitz, (Eds) Oxford: Elsevier, , pp. 1091-1102

Dorrington, RA* and JR Short (2010) The Tetraviruses. In "The Insect Viruses." K Johnson and S Asgari, (Eds). Caister Academic Press, (UK). pp 283 -  305

Published Journal Articles   (past 10 years)  

Lombard AT, Dorrington RA, Ortega-Cisneros K, Penry GS, Pichegru L, Reed JR, Smit KP, Vermeulen EA, Witteveen M, Sink KJ, Ginsburg T. Key challenges in advancing an ecosystem-based approach to marine spatial planning under economic growth imperatives. Frontiers in Marine Science. 2019;6:146. doi: 10.3389/fmars.2019.00146

Kalinski, J-CJ, Waterworth1, SC, Siwe Noundou, X, Jiwaji, M, Parker-Nance, S, Krause4, RWM, McPhail, KL, and RA Dorrington* (2019)  Molecular networking reveals two distinct chemotypes in pyrroloiminoquinone-producing Tsitsikamma favus sponges. Mar. Drugs 17(1), 60 ;

Bate, GC, Matcher, GF, Venkatachalam, S, Meiklejohn, I and Dorrington, RA. Microalgae in two freshwater lakes and an estuary as a result of groundwater contamination from households. Transactions of the Royal Society of South Africa. Accepted for publication.

Venkatachalam1, S, Matcher1,GF, Lamont, T, van den Berg, M, Ansorge, IJ and RA Dorrington* (2018) Influence of oceanographic variability on nearshore microbial communities of  the sub-Antarctic Prince Edward Islands.  Limnology and Oceanography.  Doi: 10.1002/lno.11035.

Dorrington, RA*, Lombard, AT, Bornman, TG, Adams, JB, Cawthra, HC, Deyzel, SHP, Goschen, WS, Liu, K, Mahler-Coetzee, J, Matcher, GF, McQuaid1, C, Parker-Nance, S, Paterson, A, Perissinotto, R, Porri, F, Roberts, M, Snow, B and P Vrancken (2018) Working together for our oceans: A Marine Spatial Plan for Algoa Bay, South Africa. SA J Science 114 No3-4: 18-23.

Matcher, GF, Froneman, PW, Meiklejohn, KI and RA Dorrington (2018) Distinct responses of bacterial communities to agricultural and urban impacts in temperate southern African estuaries. Estuarine, Coastal and Shelf Science 200:224-233.

Venkatachalam, S, Ansorge, IJ, Mendes, A, Melato, LI, Matcher, G. and Dorrington, RA (2017) A pivotal role for ocean eddies in the distribution of microbial communities across the Antarctic Circumpolar Current. PloS One, 12(8), p.e0183400.

Ansorge, IJ*, Skelton, P, Bekker, A, de Bruyn, PJN, Butterworth, D, Cilliers, P, Cooper, J, Cowan, DA, Dorrington, RA, Fawcett, S, Fietz, S, Findlay, KP, Froneman, PW, Grantham, GH, Greve, M, Hedding, D Hofmeyr, GJG, Kosc, M, le Roux, P, Lucas, M, MacHutchon, K, Meiklejohn, I, Nel, W, Pistorius, P, Ryan, P, Stander, J, Swart, S, Treasure, A, Vichi, M and Jansen van Vuuren, B* (2017) Exploring South Africa’s southern frontier: A 20-year vision for polar research through the South African National Antarctic Programme. South African Journal of Science 113 (5/6): 1-7.

Waterworth, SC, Jiwaji, M, Kalinski, JCJ, Parker-Nance, S and RA Dorrington* (2017) A place to call home: an analysis of the bacterial communities in two Tethya rubra Samaai and Gibbons 2005 populations in Algoa Bay, South Africa. Marine Drugs 15(4):95. doi:10.3390/md15040095

Matcher, GF, Waterworth, SC, Walmsley, TA, Matsatsa, T, Parker-Nance, S, Davies Coleman MT and RA Dorrington* (2017) Keeping it in the family: Co-evolution of latrunculid sponges and their dominant bacterial symbionts. Microbiology Open 6(2):e417. DOI: 10.1002/mbo3.417

Matcher, GF, Waterworth, SC, Walmsley, TA, Matsatsa, T, Parker-Nance, S, Davies Coleman MT and RA Dorrington* (2016) Keeping it in the family: Co-evolution of latrunculid sponges and their dominant bacterial symbionts.  Microbiology Open. Published ahead of print 26 OCT 2016 | DOI: 10.1002/mbo3.417.

Halo, I., Dorrington, R., Bornman, T., De Villiers, S. and Fawcett, S* (2016). South Africa in the Antarctic Circumnavigation Expedition: A multi-institutional and interdisciplinary scientific project. SA J Sci 112(9-10):1-4.

Ansorge, I.J., Brundrit, G., Brundrit, J., Dorrington, R., Fawcett, S., Gammon, D., Henry, T., Hermes, J., Hölscher, B., d'Hotman, J. and Meiklejohn, I.* (2016). SEAmester-South Africa's first class afloat. SA J Sci 112(9-10):.1-4.

Jiwaji, M, Short, JR and RA Dorrington* (2016) Expanding the host range of small insect RNA viruses: Providence Virus (Carmotetraviridae) infects and replicates in a human tissue culture cell line.  J.  Gen. Virol. 97: 2763-2768. doi: 10.1099/jgv.0.000578

Penkler, DL, Jiwaji, M, Domitrovic, T, Short, JR, Johnson JE and RA Dorrington* (2016)  Binding and entry of a non-enveloped T=4 insect RNA virus is triggered by alkaline pH.  Virology 498:277-287

Mendes, A, Vlok, M, Short, JR, Matsui, T and RA Dorrington* (2015)  An encapsidated viral protein and its role in RNA packaging by a non-enveloped animal RNA virus.  Virology 476:323–333.

Perissinotto, R, Bornman, TG, Steyn, P-P, Miranda, NAF, Dorrington, RA, Matcher, GF, Strydom, N and N Peer. Tufa stromatolite ecosystems on the South African south coast.  SA J. Sci. 110(9/10):1-8.

Amemiya, CT*, Dorrington, RA and A Meyer (2014) The coelacanth and its genome.  JEZ Part B: Molecular and Developmental Evolution. 322: 317-321. DOI: 10.1002/jez.b.22521

Bowker, ML, Molwantwa, JB, Gilfillin, J, Dorrington, R, Kirby, R and PD Rose* (2013) Development of a gradient tube method for examining microbial population structures in floating sulphur biofilms.  Water SA 39 (4):523-528

Matcher, GF, Jiwaji, J, de la mare, JA, and RA Dorrington* (2013) Complex pathways for regulation of pyrimidine metabolism by carbon catabolite repression and quorum sensing in Pseudomonas putida RU-KM3S.  Applied Microbiol. Biotechnol. 97: 5993-6007. doi: 10.1007/s00253-013-4862-5

Short, JR, Nakayinga, R, Walter, CT, Huhges, GE and RA Dorrington* (2013).  Providence virus (Family: Carmotetraviridae) replicates vRNA in association with the Golgi apparatus and secretory vesicles.  J. Gen. Virol. 94: 1073-1078. doi:10.1099/vir.0.048561-0

Amemiya, CT*, Alföldi, J*, Lee, AP, Fan, S, Brinkmann, H, MacCallum, I, Braasch, I, Manousaki5, T, Schneider, S, Rohner1, N, Organ1, C, Chalopin1, D, Smith, JJ, Robinson1, M, Dorrington, RA, Gerdol1, M, Aken, B, Assunta Biscotti, M, Barucca, M, Baurain1, D,  Berlin, AM, Blatch, GL, Buonocore, F Burmester, T, Campbell, MS, Canapa, A, Cannon, JP, Christoffels, A, De Moro, G, Edkins, AL, Fan, L Fausto, AM, Feiner, N, Forconi,  Gamieldien, JM, Gnerre, S,  Goldstone, JV, Haerty, W, Hahn, ME, Hesse, U, Hoffmann, S, Johnson, J, Karchner, SI, Kuraku, SI, Lara, M.   Levin, JZ, Litman, GW, Mauceli, E, Miyake, T Mueller, MG, Nelson, DR Nitsche, A,  Olmo, E, Ota, T, Pallavicini, A Panji, S Picone, B, Ponting, CP, Prohaska, SJ, Przybylski, D, Saha, NR Ravi, V, Ribeiro, FJ, Tatjana Sauka-Spengler, T, Searle, SMJ, Sharpe, T, Simakov, O, Stadler, PF, Stegeman, JJ, Sumiyama, K, Tafer, H, Turner-Maier, J, van Heusden, P,White, S, Yandell, M, Philippe, H, Volff, J-N, Tabin, CJ, Shubin, N, Schartl, M, Jaffe, D, Postlethwait, JH, Venkatesh, B, Di Palma, F, Lander, ES, Meyer, A and K Lindblad-Toh (2013) Comparative analysis of the genome of the African coelacanth, Latimeria chalumnae, sheds light on tetrapod evolution.  Nature (London) 498:311-318

Treasure, AM*, Moloney, CL, Bester, MN, McQuaid, CD, Findlay, KP, Best, PB, Cowan, DA, de Bruyn, PJN, Dorrington, RA, Fagereng, A, Froneman, PW, Grantham, GH, Hunt, BPV, Meiklejohn, KI, Pakhomov, EA, Roychoudhury, AN, Ryan, PG, Smith, VR, Chown, SL and IJ Ansorge. (2013) South African research in the Southern Ocean: new opportunities and challenges.  SA J Sci. 109 (3/4): 1-4

Short, JR and RA Dorrington* (2012) Membrane targeting of an alpha-like tetravirus replicase is directed by a region within the RNA-dependent RNA polymerase domain.  J. Gen. Virol. 93:1706 - 1716

Walmsley, TA, Matcher, GF, Zhang, F, Hill, RT, Davies-Coleman, MT and RA Dorrington * (2012) Diversity of bacterial communities associated with the Indian Ocean sponge Tsitsikamma favus that contains the bioactive pyrroloiminoquinones, Tsitsikammamine A and B.  Marine Biotechnol. 14(6): 681-691.  DOI 10.1007/s10126-012-9430-y

Matcher, GF*, Dorrington, RA Henninger, TO and PW Froneman (2011) A preliminary study on the microbial diversity in a freshwater-deprived permanently open Eastern Cape estuary, using 16S rRNA pyrosequencing analysis.  Water SA. 37 (3): 381-389

Walter, CT, Pringle, FM, Nakayinga, R, de Felipe, P, Ryan, MD, Ball, LA and RA Dorrington, * (2010)  Genome organization and translation products of Providence virus: insight into a unique tetravirus.  J. Gen. Virol.  91(11): 2826-35

Short, JR, Knox, C and RA Dorrington* (2010) Subcellular localisation and live cell imaging of the Helicoverpa armigera stunt virus replicase in mammalian and Spodoptera frugiperda cells. J Gen Virol; 91 (6): 1514 – 1523               

Jiwaji, M and RA Dorrington* (2009) Regulation of hydantoin-hydrolyzing enzyme expression in Agrobacterium tumefaciens strain RU-AEO1.  Appl. Microbiol. Biotechnol.  84(6):1169-79

Modisakeng, KW, Jiwaji, M, Pesce, E-R, Robert, J, Amemiya, CT, Dorrington, RA and GL Blatch * (2009)  Isolation of a Latimeria menadoensis heat shock protein 70 Lmhsp70) that has all the features of an inducible gene and encodes a functional molecular chaperone.  Mol Genet Genomics.  ;282(2):185-96.

Jiwaji, M, Hartley, CJ, Clark, S-A, Burton, SB and RA Dorrington* (2009) Enhanced hydantoin-hydrolyzing enzyme activity in an Agrobacterium tumefaciens strain with two distinct N-carbamoylases.  Enzyme Microb. Technol. 44 (2009) 203–209

Gentz PM, Blatch, GL and RA Dorrington* (2009).  Dimerisation of the yeast eukaryotic translation initiation factor 5A requires hypusine and is RNA dependent. FEBS J. 276 (3):695-706

Jiwaji, M, Matcher, GF and Dorrington RA,* (2008)  A broad host range reporter plasmid for analysis of divergent promoter regions.  Sf J Sci 104 (7-8), 305-307

Walter, CT, Tomasicchio, M, Hodgson, V, Hendry, DA, Hill, MP and RA Dorrington (2008) Characterisation of a succession of small insect viruses in a wild South African population of Nudaurelia cytherea capensis. SA J. Sci. 104 (3-4):147-152

Kirchmann, S, van Zyl, P, Brady D*, Abrahams, N, Rech, S, Dorrington, RA and SG Burton (2007). A dual phase fermentation protocol for the production of hydantoinase and carbamoylase by the wild type Pseudomonas putida RU-KM3.  Enzymes and Microbial Technology, 41: 539-545

Tomasicchio, M, Venter, PA, Gordon, KHJ, Hanzlik, TN and RA Dorrington* (2007).  Induction of apoptosis in Saccharomyces cerevisiae results in the spontaneous maturation of tetravirus procapsids in vivo.  J Gen Virol; 88: 1576-1582

Bulawayo, B, Dorrington, RA and SG Burton* (2007).  Enhanced operational parameters for amino acid production using hydantoin-hydrolysing enzymes of Pseudomonas putida strain RUKM3s. Enzyme and Microbial Technology 40 (4):533-539                        

Modisakeng, KW, Amemiya, CT, Dorrington, RA* and GL Blatch* (2006).  Molecular studies on the coelacanth: A Review.  S. A. J. Sci.  102: 479-485.

du Plessis L, Hendry DA, Dorrington, RA, Hanzlik TN, Johnson JE, and M Appel*. (2005). Revised RNA2 sequence of the tetravirus, Nudaurelia capensis Omega virus (NwV). Arch. Virol. 150(11): 2397-2402     

Publications (2004 and before)

Modisakeng, K.W., Dorrington, RA and GL Blatch* (2004). Isolation of genes encoding heat shock protein 70 (hsp70s) from the coelacanth,  Latimeria chalumnae. Special Rhodes University Centenary Issue of S.  Afr. J. Sci. 100: 683-686

Burton, SG* and RA Dorrington (2004). Hydantoin-hydrolysing enzymes for enantioselective production of amino acids – new insights and applications.  Tetrahedron: Asymmetry, 15 (18): 2737-2741                                                       

Atomi, H, Imanaka, T, Burton, S., and RA Dorrington (2004). Integrating biocatalysis into organic synthesis. Tetrahedron: Asymmetry, 15(18).

Matcher, GF, Burton, SG and RA Dorrington * (2004). Mutational analysis of the hydantoin-hydrolysis pathway in Pseudomonas putida RU-KM3S.  Applied Microbiology and Biotechnology 65 (4): 391 - 400                                    

Foster, IM, Dorrington, RA and SG. Burton* (2003).  Enhanced hydantoinase and N-carbamoylase activity on immobilisation of Agrobacterium tumefaciens strain RU-ORPN1.  Biotechnol. Lett.  25: 67-72                                          

Idicula, AM, Blatch, GL, Cooper TG and RA Dorrington * (2002). Binding and activation by the Zn cluster transcription factors of Saccharomyces cerevisiae: redefining the UASGABA and its interaction with Uga3p. J Biol Chem. 277(48):45977-83                         

Hide W*, Mizrahi V, Venkatesh B, Brenner S, Simpson A, Blatch G, Soodyall H, Denby K, Wingfield M,Wingfield B, van Helden P, Ramesar R, Dorrington, RA, Kelso J, Oppon E, Goyvaerts E, Ramsay M, de Villiers E, van Heerden C, Allsopp B and C Seoighe (2001).  A platform for genomics in South Africa.  S Afr Med J. 2001 Dec; 91(12):1006-7   

Hartley, CJ, Manford, F, Burton, SG and RA Dorrington* (2001).  Overproduction of hydantoinase and N-carbamoylase enzymes by regulatory mutants of Agrobacterium tumefaciens.  Appl Microbiol Biotechnol (2001) 57: 43-49                 

Buchanan K, Burton SG*, Dorrington, RA, Matcher GM and Z Skepu. (2001). A novel Pseudomonas putida strain with high levels of hydantoin-converting activity, producing l-amino acids. J. Molec. Catal. B (Enzymic) 11, 397- 406

Scott S, Dorrington, RA, Svetlov V, Beeser AE, Distler M and TG Cooper*  (2000).  Functional Domain Mapping and Subcellular Distribution of Dal82p in Saccharomyces cerevisiae.  J Biol Chem.; 275(10):7198-7204                   

Rein, N, Dorrington, RA, Lewis, A, Lowenthal, R, and PD Rose* (2000).  The sulphide oxidizing biofilm reactor (SOBR): A component unit operation of the Rhodes SURE process for sewage sludge solubilization.  Chemical Technology, March/April 2001: 14 – 16

Park, H-D, Scott, S, Rai, R, Dorrington, RA and TG Cooper * (1999). Synergistic Operation of the CAR2 (Ornithine Transaminase) Promoter Elements in S. cerevisiae. J. Bacteriol. 181:7052-7064

Burton*, SG, Dorrington, RA, Hartley, CJ, Kirchmann, S, Buchanan, K and V Phehane (1998).  Production of enantiomerically pure amino acids: characterisation of South African hydantoinases and hydantoinase-producing bacteria. J. Molec. Catal. (B: Enzymic) 5: 301 – 305                       

Hartley, CH, Kirchmann, S, Burton, SG and RA Dorrington* (1998)  Characterisation of hydantoin hydrolysis by Agrobacterium tumefaciens RU-OR and isolation of a novel constitutive mutant. Biotechnol. Lett. 20 (7): 707-7              

Cunningham, TC, Dorrington, RA and TG Cooper* (1994) The UGA4 UISNTR site required for GLN3-dependent transcriptional activation also mediates DAL80-responsive regulation and DAL80 protein binding. J. Bacteriol. 76:4718-4725

Dorrington, RA and TG Cooper* (1993) The DAL82 Protein of Saccharomyces cerevisiae binds to the DAL upstream inducer sequence (UIS). Nuc. Acids Res. 21: 3777-3784     

Rawlings. DE*, Dorrington, RA, Rohrer, J and A-M Clennel (1993) A molecular analysis of a broad-host-range plasmid isolated from Thiobacillus ferrooxidans.  FEMS Microbiol. Reviews 11:3-8

Dorrington, RA, Bardiem, S and DE Rawlings * (1991) The minimum replicon of broad-host-range plasmid pTF-FC2 requires a primase-like protein for replication in E. coli. Gene 108: 7-14           

Dorrington, RA and Rawlings, DE* (1990)  Characterisation of the minimum replicon of the broad-host-range plasmid pTF-FC2 and its similarity to the incoQ plasmids. J. Bacteriol. 172:5697-5705

Dorrington, RA and DE Rawlings* (1989) Identification and sequence of the basic replication region of a broad-host-range plasmid isolated from Thiobaciluus ferrooxidans. J. Bacteriol. 171:2735-2739

Dorrington, RA and DR Woods* (1986) Cloning of a T-DNA homologous fragment from Protea cynaroides. S.A.J. Sci. 82 (8):453-454  


Marine natural products research and microbial ecology

  • Prof Heinrich Hoppe (Dept of Biochemistry and Microbiology, Rhodes University)
  • Prof Rui Krause  (Dept of Chemistry, Rhodes University)
  • Dr Tommy Bornman (Ewandile Node, South African Environmental Observation Noted)
  • Dr Angus Patterson and Mr Roger Bills (South African Institute for Aquatic Biodiversity )
  • Prof Mike Davies Coleman (Dean of Science, Department of Chemistry, University of the Western Cape)
  • Prof Renzo Perissinotto (Dept of Zoology, Nelson Mandela Metropolitan University)
  • Dr Kerry McPhail  (School of Pharmacology, Oregon State University)

Insect RNA viruses and the development of antiviral therapies

  • Prof Martin Hill (Dept of Zoology and Entomology, Rhodes University)
  • Prof Jack Johnson (Dept of Molecular Biology, The Scripps Institute)
  • Dr Tatiana Domitrovic (Federal University of Rio de Janeiro)

Microbial ecology of extreme environments

  • Prof Ian Meiklejohn (Dept of Geography, Rhodes Unviersity
  • Prof Bettine Jansen van Vuuren (Dept of Zoology, University of Johannesburg)
  • Prof William Froneman (Dept of Zoology and Entomology, Rhodes Unviersity)

Last Modified: Mon, 27 May 2019 09:05:18 SAST